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A method for determining supernatant-free spectra generated from fluorescently stained cells in suspension

Paper ID Volume ID Publish Year Pages File Format Full-Text
29848 44444 2014 6 PDF Available
Title
A method for determining supernatant-free spectra generated from fluorescently stained cells in suspension
Abstract

•We present a cuvette holder for the front-face fluorometry of pelleted cell layers.•Supernatant-free fluorescence spectra of cells in suspension were measured.•Yeast cells stained with calcofluor and 3,3′-dipropylthiadicarbocyanine were used.•The presented method is also suitable for any other cell type and fluorescent dye.

Here we present a fluorometric method for direct determination of supernatant-free fluorescence spectra generated from fluorescently stained cells in suspension. The key element in the new technique is the design of an adapter to a standard cuvette holder that makes it possible to measure front-face fluorescence spectra from thin layers of cells spun down to the bottom of a spectrofluorometric cuvette. We have demonstrated the applicability of this approach and its analytical potential using the suspensions of yeast cells stained with the potentiometric dye of 3,3’-dipropylthiadicarbocyanine, diS-C3(3), and with the specific cell-wall marker calcofluor.

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A method for determining supernatant-free spectra generated from fluorescently stained cells in suspension
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 141, December 2014, Pages 139–144
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us