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Mechanical loading regulates human MSC differentiation in a multi-layer hydrogel for osteochondral tissue engineering

Paper ID Volume ID Publish Year Pages File Format Full-Text
299 23 2015 12 PDF Available
Title
Mechanical loading regulates human MSC differentiation in a multi-layer hydrogel for osteochondral tissue engineering
Abstract

A bioinspired multi-layer hydrogel was developed for the encapsulation of human mesenchymal stem cells (hMSCs) as a platform for osteochondral tissue engineering. The spatial presentation of biochemical cues, via incorporation of extracellular matrix analogs, and mechanical cues, via both hydrogel crosslink density and externally applied mechanical loads, were characterized in each layer. A simple sequential photopolymerization method was employed to form stable poly(ethylene glycol)-based hydrogels with a soft cartilage-like layer of chondroitin sulfate and low RGD concentrations, a stiff bone-like layer with high RGD concentrations, and an intermediate interfacial layer. Under a compressive load, the variation in hydrogel stiffness within each layer produced high strains in the soft cartilage-like layer, low strains in the stiff bone-like layer, and moderate strains in the interfacial layer. When hMSC-laden hydrogels were cultured statically in osteochondral differentiation media, the local biochemical and matrix stiffness cues were not sufficient to spatially guide hMSC differentiation after 21 days. However dynamic mechanical stimulation led to differentially high expression of collagens with collagen II in the cartilage-like layer, collagen X in the interfacial layer and collagen I in the bone-like layer and mineral deposits localized to the bone layer. Overall, these findings point to external mechanical stimulation as a potent regulator of hMSC differentiation toward osteochondral cellular phenotypes.

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Keywords
Hydrogel; Human mesenchymal stem cell; Cellular strain; Dynamic mechanical loading; Osteochondral tissue engineering
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Mechanical loading regulates human MSC differentiation in a multi-layer hydrogel for osteochondral tissue engineering
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 21, 15 July 2015, Pages 142–153
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us