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Synergistic effect of photodynamic therapy and cisplatin: A novel approach for cervical cancer

Paper ID Volume ID Publish Year Pages File Format Full-Text
29964 44448 2014 9 PDF Available
Title
Synergistic effect of photodynamic therapy and cisplatin: A novel approach for cervical cancer
Abstract

•We studied anticancer potential of PDT individually and combined.•We examined PDT action using methylene blue or Photogem on cervical cancer cells.•We examined cisplatin action as monotherapy and combined with PDT.•Results of combined therapy was significantly better than monotherapies.•The sequence of treatments (PDT + cisplatin/cisplatin + PDT) affected the results.

Cervical cancer is a neoplasia primarily caused by Human papillomavirus (HPV) infection. Current treatment modalities involve cisplatin, a potent chemotherapeutic agent with severe adverse effects. Photodynamic therapy (PDT) is a promising modality for the treatment of cancer and infections, which has been associated with innovative therapeutic approaches, especially for the treatment of neoplasias. This study aimed to investigate the anticancer potential of PDT mediated by methylene blue (MB) or Photogem (PG) individually and combined with cisplatin in vitro. SiHa, C-33 A and HaCaT cells were incubated with MB, PG and/or cisplatin and received no further treatment or were irradiated with a 630 or a 660 nm LED light source at energy densities varying according to the photosensitizer (PS). The MTT assay was employed to assess cell viability. Both PS were effective in reducing cell viability with the cytotoxicity being dependent on the light dose. When compared to PDT groups, cisplatin was less effective. The cell viability of the combined therapy groups was significantly lower compared to monotherapies. The sequence of treatments (PDT + cisplatin/cisplatin + PDT) was important and had different results when varying the PS, but combination therapy resulted in an enhanced anticancer effect regardless of treatment protocol.

Keywords
Photodynamic therapy; Cisplatin; Cervical cancer; Combined therapy
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 140, November 2014, Pages 365–373
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us