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Specific interaction of 4′-O-(a-l-Cladinosyl) daunorubicin with human serum albumin: The binding site II on HSA molecular using spectroscopy and modeling

Paper ID Volume ID Publish Year Pages File Format Full-Text
29973 44449 2009 8 PDF Available
Title
Specific interaction of 4′-O-(a-l-Cladinosyl) daunorubicin with human serum albumin: The binding site II on HSA molecular using spectroscopy and modeling
Abstract

This study was designed to examine the interaction of 4′-O-(a-l-Cladinosyl) daunorubicin (CDNR), a disaccharide anthracycline with potent antitumor activity against leukemia cell line K562 cells and colon cancer cell line SW620 cells, with human serum albumin (HSA) for the first time by fluorescence spectroscopy in combination with UV-absorption and molecular modeling under simulative physiological conditions. The quenching mechanism was suggested to be static quenching according to the fluorescence measurement. The thermodynamic parameters, enthalpy change (ΔH) and entropy change (ΔS) were calculated to be −19.89 KJ/mol and 26.23 J/mol/K, according to the Van’t Hoff equation. These data suggested that hydrophobic interaction was the predominant intermolecular forces stabilizing the complex, which was in good agreement with the results of molecular modeling study. The effect of CDNR on the conformation of HSA was analyzed using synchronous fluorescence spectroscopy and UV/vis absorption spectroscopy. In addition, the effects of common ions on the binding constant of CDNR–HSA complex were also discussed at room temperature.

Keywords
4′-O-(a-l-Cladinosyl) daunorubicin; Human serum albumin (HSA); Fluorescence quenching; Molecular modeling; Synchronous fluorescence
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Specific interaction of 4′-O-(a-l-Cladinosyl) daunorubicin with human serum albumin: The binding site II on HSA molecular using spectroscopy and modeling
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 95, Issue 3, 3 June 2009, Pages 162–169
Authors
, , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us