fulltext.study @t Gmail

An overview of structural features of DNA and RNA complexes with saffron compounds: Models and antioxidant activity

Paper ID Volume ID Publish Year Pages File Format Full-Text
29979 44449 2009 9 PDF Available
Title
An overview of structural features of DNA and RNA complexes with saffron compounds: Models and antioxidant activity
Abstract

Saffron is the red dried stigmas of Crocus sativus L. flowers and used both as a spice and as a drug in traditional medicine. Its numerous applications as an antioxidant and anticancer agent are due to its secondary metabolites and their derivatives (safranal, crocetin, dimethylcrocetin). In this work we are comparing the spectroscopic results and antioxidant activities of saffron components safranal, crocetin (CRT) and dimethylcrocetin (DMCRT) complexes with calf-thymus DNA (ctDNA) and transfer RNA (tRNA) in aqueous solution at physiological conditions Intercalative and external binding modes of saffron compounds to DNA and RNA were observed with overall binding constants of Ksafranal = 1.24 × 103 M−1, KCRT = 6.20 × 103 M−1 and KDMCRT = 1.85 × 105 M−1, for DNA adducts and Ksafranal = 6.80 × 103 M−1, KCRT = 1.40 × 104 M−1 and KDMCRT = 3.40 × 104 M−1 for RNA complexes. A partial B- to A-DNA transition occurred at high ligand concentrations, while tRNA remained in A-conformation in saffron–RNA complexes. The antioxidant activity of CRT, DMCRT and safranal was also tested by the DPPH˙ (2,2-diphenyl-1-picrylhydrazyl) antioxidant activity assay and their IC50 values were compared to that of well known antioxidants such as Trolox and Butylated Hydroxy Toluene (BHT). The IC50 values were 95 ± 1 μg/mL for safranal and 18 ± 1 μg/mL for crocetin. The inhibition of DMCRT reached a point of 38.8%, which corresponds to a concentration of 40 μg/mL.

Keywords
Saffron; DNA; tRNA; Antioxidant; Conformation; FT-IR
First Page Preview
An overview of structural features of DNA and RNA complexes with saffron compounds: Models and antioxidant activity
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 95, Issue 3, 3 June 2009, Pages 204–212
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us