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In vitro photodynamic antimicrobial activity of methylene blue and endoscopic white light against Helicobacter pylori 26695

Paper ID Volume ID Publish Year Pages File Format Full-Text
29986 44451 2010 4 PDF Available
Title
In vitro photodynamic antimicrobial activity of methylene blue and endoscopic white light against Helicobacter pylori 26695
Abstract

Photodynamic therapy (PDT) is a method for inactivating cells (viral, bacterial and cancer cells) using photosensitizers (PS) and light of various wavelengths. Helicobacter pylori might be easily affected by light because it has few genes to repair light-induced DNA damage. In vitro PDT against H. pylori was conducted using endoscopic white light and methylene blue (MB) as the PS before application to in vivo study. The bactericidal effects were measured by counting viable cells after PDT. The degree of oxidative damage of DNA was confirmed using alkaline gel electrophoresis, real-time PCR (RT-PCR) and an assay of 8-hydroxy-2-deoxyguanosine (8-OHdG). In the control group, the number of viable cells was maintained constantly during the experiment. In the groups treated with either 0.2 mg/ml MB alone or white light with 0.02 mg/ml MB for 10 min, bacteria decreased approximately a hundredfold. The killing effect increased proportionally to the PS concentration and the duration of irradiation. DNA damage by PDT proven by alkaline gel electrophoresis, RT-PCR and assay of 8-OHdG, was greater in PDT-treated groups than in control. PDT using MB and endoscopic white light showed effective bactericidal activity in vitro by oxidative DNA damage of H. pylori.

Keywords
Photodynamic therapy; Methylene blue; Endoscopy; Helicobacter pylori
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In vitro photodynamic antimicrobial activity of methylene blue and endoscopic white light against Helicobacter pylori 26695
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 101, Issue 3, 2 December 2010, Pages 206–209
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us