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Novel fluorescent conjugate containing glucose and NBD and its carrier-mediated uptake by tobacco cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
29988 44451 2010 9 PDF Available
Title
Novel fluorescent conjugate containing glucose and NBD and its carrier-mediated uptake by tobacco cells
Abstract

Some compounds that contain glucose groups can be transported across the plasma membrane into the cells through hexose transporters. To test the hypothesis that glucose-conjugated insecticides also have similar uptake and translocation properties, a novel fluorescent conjugate (12) was prepared by conjugating glucose and 7-nitrobenz-2-oxa-1,3-diazole with 4-iodo-1-phenylpyrazoles. Its fluorescence spectra and uptake by suspension-cultured tobacco (Nicotiana tabacum L.cv.) cells were studied. The fluorescence spectra showed long wavelengths with maximum emission at 530 nm. After incubating tobacco cell suspensions in 10 μM conjugate for 0.5 h, green fluorescence of 12 was clearly visible in the cells under fluorescence microscopy. After 2 h of incubation, more than 70% of 12 was absorbed. Carbonyl cyanide m-chlorophenylhydrazone, phloridzin and glucose drastically inhibited uptake. In concentration-dependent uptakes, the uptake rate of 12 showed a saturable component and was in accordance with Michaelis–Menten kinetics. The results proved that the glucose moiety can guide 12 into tobacco cells and that hexose transporters mediated the uptake.

Keywords
Glucose; Insecticide; Hexose transporter; Fluorescence
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Novel fluorescent conjugate containing glucose and NBD and its carrier-mediated uptake by tobacco cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 101, Issue 3, 2 December 2010, Pages 215–223
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us