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The role of nitric oxide in the treatment of tumours with aminolaevulinic acid-induced photodynamic therapy

Paper ID Volume ID Publish Year Pages File Format Full-Text
29989 44451 2010 9 PDF Available
Title
The role of nitric oxide in the treatment of tumours with aminolaevulinic acid-induced photodynamic therapy
Abstract

Photodynamic therapy (PDT) is a local cancer treatment which induces cell death by the interaction of light with a photosensitizing drug. Previous studies indicate that nitric oxide (NO) plays a role in Photofrin-PDT, but this has not been investigated in aminolaevulinic acid (ALA)-PDT. The current study determines whether inhibition of nitric oxide synthase (NOS) activity modulates treatment responses to ALA-PDT, in tumours displaying differential levels of NO.Murine tumours with low (EMT6) or high (RIF-1) NO levels were implanted into the cremaster muscle of BALB/c or C3H/HeN mice respectively. Animals were prepared for in vivo microscopy 7–14 days later. Mice received oral ALA (200 mg/kg) 4 h before PDT. l-NAME, l-NNA or 1400 W (10 mg/kg) were administered via the jugular vein 5 min before PDT.NOS inhibition (l-NAME or l-NNA) combined with ALA-PDT in RIF-1 tumours demonstrated enhanced damage to both the tumour and normal microvasculature, with increased macromolecular leak and reduction in vessel diameter, whereas ALA-PDT alone had no effect. In contrast, EMT6 tumours responded to ALA-PDT alone but sensitivity was not enhanced in the presence of NOS inhibition. 1400 W combined with ALA-PDT induced similar microvascular effects to l-NAME in both tumours, but were less pronounced.The data demonstrates that NO has an important role in events likely to be critical for treatment response, sensitivity and therapeutic outcome of ALA-PDT.

Keywords
Aminolaevulinic acid; Photodynamic therapy; Nitric oxide; Nitric oxide synthase inhibitors; In vivo microscopy; Tumours
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 101, Issue 3, 2 December 2010, Pages 224–232
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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