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Analysis of in vivo chlorophyll fluorescence spectra to monitor physiological state of tomato plants growing under zinc stress

Paper ID Volume ID Publish Year Pages File Format Full-Text
30002 44451 2010 8 PDF Available
Title
Analysis of in vivo chlorophyll fluorescence spectra to monitor physiological state of tomato plants growing under zinc stress
Abstract

The effects of zinc (Zn) on plant chlorophyll fluorescence were investigated in 10-day-old tomato (Solanum lycopersicum) seedlings subjected for 7 days to a series of zinc (10, 50, 100 and 150 μM) applied via the nutrient solution. The chlorophyll fluorescence spectra of leaves were recorded in the spectral region 650–800 nm using the spectroscopic technique of ultraviolet light emitting diode induced fluorescence spectroscopy (UV-LED IFS). These spectra have been used to analyze the effect of several doses of zinc on the photosynthetic activities of tomato plants. The fluorescence intensity ratios (FIR) at the two maxima (F690/F735) of control as well as treated tomato plants were calculated by evaluating curve-fitted parameters using a Gaussian spectral function. The variable chlorophyll fluorescence decrease ratio (RFd) values were determined from the fluorescence induction kinetics curves recorded at 690 nm and 735 nm. In addition, Zn accumulation in plants, plant growth, photosynthetic pigments content and malondialdehyde level (MDA, an index of lipid peroxidation) were also estimated. The results indicated that the plants treated with 10 μM of zinc exhibited better growth, however, higher concentrations of zinc were harmful for plants. Excess Zn induced a decrease in the RFd values, which was associated with a strong decline of the total chlorophylls content and an increase of MDA level. The total chlorophylls content decline could also be followed via an increase of the chlorophyll fluorescence ratio F690/F735.

Keywords
Chlorophyll fluorescence; Plant; Stress; Zinc
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Analysis of in vivo chlorophyll fluorescence spectra to monitor physiological state of tomato plants growing under zinc stress
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 101, Issue 3, 2 December 2010, Pages 332–339
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us