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Increasing photostability and water-solubility of carotenoids: Synthesis and characterization of β-carotene–humic acid complexes

Paper ID Volume ID Publish Year Pages File Format Full-Text
30005 44451 2010 7 PDF Available
Title
Increasing photostability and water-solubility of carotenoids: Synthesis and characterization of β-carotene–humic acid complexes
Abstract

Carotenoids are a group of phytochemicals that are recognized as playing an important role in the prevention of several degenerative diseases due to their antioxidant activity. The low water-solubility and poor photostability of β-carotene, a potentially important dietary antioxidant, prompted us to investigate β-carotene complexes able to increase its water solubility as well as its photostability in aqueous solution. In this work we have used humic acid in order to synthesize β-carotene–humic acid complexes in different ratios.Complexes were characterized by FT-IR and DSC. β-Carotene determination were performed by HPLC and antioxidant activity of β-carotene, humic acid and the complexes were determined by the TEAC method. The formulation β-carotene–humic acid 2:1 showed the highest photostability and water solubility. The antioxidant activity of the complexes was about 20 times lower than “free” β-carotene, suggesting a decreased chemical reactivity of the carotenoid in the complex. We showed that complexation of β-carotene with humic acids was able to increase its water solubility and photostability, and to affect the antioxidant activity of β-carotene after inclusion in HAs. This work provides a useful tool to avoid antioxidants photodegradation and oxidation by radicals, thus maintaining their biological activity.

Keywords
β-Carotene; Photosensitivity; Humic acid; Complexes; Water solubility
First Page Preview
Increasing photostability and water-solubility of carotenoids: Synthesis and characterization of β-carotene–humic acid complexes
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 101, Issue 3, 2 December 2010, Pages 355–361
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering