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Photophysical properties of a new DyLight 594 dye

Paper ID Volume ID Publish Year Pages File Format Full-Text
30035 44456 2010 5 PDF Available
Title
Photophysical properties of a new DyLight 594 dye
Abstract

We describe spectral properties of novel fluorescence probe DyLightâ„¢ 594. Absorption and fluorescence spectra of this dye are in the region of Alexa 594 fluor spectra. The quantum yield of DyLight 594 in conjugated form to IgG is higher than corresponding quantum yield of Alexa 594 by about 50%. The new DyLight dye also shows slightly longer lifetime and photostability. These favorable properties and high anisotropy value, as well as a high cross-section for two-photon excitation, make this fluorophore attractive as a fluorescence probe in biochemical/biological studies involving fluorescence methods.

Keywords
Fluorophore; Quantum yield; Anisotropy; Fluorescence lifetime
First Page Preview
Photophysical properties of a new DyLight 594 dye
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 98, Issue 1, 21 January 2010, Pages 35–39
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering