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In vitro human cell responses to a low-dose photodynamic treatment vs. mild H2O2 exposure

Paper ID Volume ID Publish Year Pages File Format Full-Text
30048 44457 2015 8 PDF Available
Title
In vitro human cell responses to a low-dose photodynamic treatment vs. mild H2O2 exposure
Abstract

•Low-dose short-time ROS exposure increases in vitro human cell proliferation.•The observed mitotic enhancement is transient after ROS exposure.•H2O2 at low concentration greatly increases micronuclei frequency.•Low-dose PDT treatment does not induce micronuclei increase.

Photodynamic treatments allow control of the amount of reactive oxygen species (ROS) produced through the photosensitizer concentration and the light dose delivered to the target. In this way low ROS doses can be achieved in situ to study cell responses related to redox regulation. In this study a comparison has been made between different cell responses to a low-dose photodynamic treatment and both low and relatively high concentrations of H2O2 in human immortalized keratinocytes (HaCaT). The obtained results show that the photodynamic treatment induces a stimulating cell response roughly equivalent to that produced by exposing cells to 10−5 M H2O2. Higher H2O2 concentrations gave rise to concentration-dependent deleterious effects on the cell cultures. Of importance is that the photodynamic treatment did not produce genotoxic damage, as measured by micronuclei frequency, while cultures exposed to 10−5 M H2O2 displayed a significant increase in the amount of cells with micronuclei. In summary, the low-dose photodynamic treatment promotes cell proliferation but does not incur in the excessive clastogenic lesions observed after H2O2 exposure. It is therefore proposed as a promising alternative to direct H2O2 exposure in the study of cell redox signalling.

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In vitro human cell responses to a low-dose photodynamic treatment vs. mild H2O2 exposure
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 143, February 2015, Pages 12–19
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us