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Spectroscopy, calorimetry and molecular simulation studies on the interaction of catalase with copper ion

Paper ID Volume ID Publish Year Pages File Format Full-Text
30058 44457 2015 7 PDF Available
Title
Spectroscopy, calorimetry and molecular simulation studies on the interaction of catalase with copper ion
Abstract

•The binding stoichiometry of copper ion to catalase is 11.4.•The result of catalase activity on molecular level was consistent with that in the cellular test.•The conformation and function of catalase were changed induced by copper ion.•This study established a combined cellular and molecular toxicity evaluation method.

In this research, the binding mechanism of Cu2+ to bovine liver catalase (BLC) was studied by fluorescence spectroscopy, ultraviolet–visible (UV–vis) absorption spectroscopy, circular dichroism (CD) spectroscopy, isothermal titration calorimetry (ITC) and molecular docking methods. The cellar experiment was firstly carried out to investigate the inhibition effect of catalase. During the fluorescence quenching study, after correcting the inner filter effect (IFE), the fluorescence of BLC was found to be quenched by Cu2+. The quenching mechanism was determined by fluorescence lifetime measurement, and was confirmed to be the dynamic mode. The secondary structure content of BLC was changed by the addition of Cu2+, as revealed by UV–vis absorption and CD spectra, which further induces the decrease in BLC activity. Molecular simulation study indicates that Cu2+ is located between two β-sheets and two random coils of BLC near to the heme group, and interacts with His 74 and Ser 113 residues near a hydrophilic area. The decrease of α-helix and the binding of His 74 are considered to be the major reason for the inhibition of BLC activity caused by Cu2+. The ITC results indicate that the binding stoichiometry of Cu2+ to catalase is 11.4. Moreover, the binding of Cu2+ to BLC destroyed H-bonds, which was confirmed by the CD result.

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Spectroscopy, calorimetry and molecular simulation studies on the interaction of catalase with copper ion
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 143, February 2015, Pages 100–106
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us