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Photodynamic inactivation of Gram-positive bacteria employing natural resources

Paper ID Volume ID Publish Year Pages File Format Full-Text
30088 44459 2014 10 PDF Available
Title
Photodynamic inactivation of Gram-positive bacteria employing natural resources
Abstract

•We screened a collection of Argentinian plants as photosensitizers for bacteria.•We identified 3 extracts as highly photoactive against S. epidermidis.•Solanum verbascifolium extract was the most photoactive.•S. verbascifolium – Photoinactivation was highly efficient under sunlight.•Natural resources can be employed in Photoinactivation of bacteria.

The aim of this paper was to investigate a collection of plant extracts from Argentina as a source of new natural photosensitizers (PS) to be used in Photodynamic Inactivation (PDI) of bacteria. A collection of plants were screened for phototoxicity upon the Gram-positive species Staphylococcus epidermidis.Three extracts turned out to be photoactive: Solanum verbascifolium flower, Tecoma stans flower and Cissus verticillata root. Upon exposure to a light dose of 55 J/cm2, they induced 4, 2 and 3 logs decrease in bacterial survival, respectively.Photochemical characterisation of S. verbascifolium extract was carried out. PDI reaction was dependent mainly on singlet oxygen and to a lesser extent, on hydroxyl radicals, through type II and I reactions. Photodegradation experiments revealed that the active principle of the extract was not particularly photolabile.It is noticeable that S. verbascifolium –PDI was more efficient under sunlight as compared to artificial light (total eradication vs. 4 logs decrease upon 120 min of sunlight). The balance between oxidant and antioxidant compounds is likely to be masking or unmasking potential PS of plant extracts, but employing the crude extract, the level of photoactivity of S. verbascifolium is similar to some artificial PS upon exposure to sunlight, demonstrating that natural resources can be employed in PDI of bacteria.

Keywords
DMSO, dimethyl sulphoxide; MTT, (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazoliumbromide; PDT, photodynamic therapy; PDI, photodynamic inactivation; PS, photosensitizerBacteria; Natural products; Photoinactivation; Photodynamic therapy; Plants; Sunlig
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Photodynamic inactivation of Gram-positive bacteria employing natural resources
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 133, 5 April 2014, Pages 80–89
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us