Evaluation of the effects of systemic photodynamic therapy in a rat model of acute myeloid leukemia
•SPDT was performed on blood from BNML rats during extracorporeal circulation.•SPDT increased the survival time of BNML rats.•BNML rats subjected to SPDT exhibited reduced numbers of leukemia cells.•BNML rats subjected to SPDT exhibited reduced serum cytokine levels.
Systemic PDT (SPDT) approach is developed to treat a variety of hematological diseases, including cancers and blood-borne infections. We evaluated the efficacy of an SPDT method for treating leukemia using a Brown Norway myeloid leukemia (BNML) rat model with the LT12 cells engineered to express GFP. The survival times of animals receiving SPDT at 5 (early-SPDT) and 10 (mid-SPDT) days post-LT12 injection were prolonged by 2 days, the rats in the late-SPDT group (15 days) exhibited a 6-day increase in life span (p < 0.05). The percentages of GFP-LT12 cells in the bone marrow of the late-SPDT rats decreased from 61.6% to 56.5% on day 17. Likewise, there was a decrease in the serum expression levels of IL-1β, IL-10, TNF-α, and IFN-γ in the late-SPDT rats (p < 0.05). Our findings indicate that SPDT could be an effective method for the treatment of leukemia, and that antitumor immunity may play a key role in this process.
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 153, December 2015, Pages 13–19