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Synthesis, characterization and in vitro evaluation of methotrexate conjugated fluorescent carbon nanoparticles as drug delivery system for human lung cancer targeting

Paper ID Volume ID Publish Year Pages File Format Full-Text
30111 44460 2015 10 PDF Available
Title
Synthesis, characterization and in vitro evaluation of methotrexate conjugated fluorescent carbon nanoparticles as drug delivery system for human lung cancer targeting
Abstract

•Fluorescent carbon nanoparticles were synthesized from glucose by ultrasonication.•Carbon nanoparticles were characterized by microscopic and spectroscopic techniques.•Functionalized carbon nanoparticles were attached with anti cancer drug-methotrexate.•Bioactivity of CNPs and CNPs-MTX conjugates was evaluated by biological endpoints.•CNPs-MTX conjugates showed promising cytotoxicity against lung carcinoma cell line.

Nanotechnology based cancer therapeutics have rapidly advanced towards the solution of many limitations associated with other drug delivery agents such as nonspecific distribution within the body, low water solubility and non-biocompatibility. Carbon nanoparticles have demonstrated unique properties that are useful to combat with these issues, including their properties dependent on size, high stability in different solvents, compatible size for drug delivery and ease of surface modifications. Fluorescent carbon nanoparticles with good water solubility were obtained from a carbohydrate source by acid assisted ultrasonic treatment at 35 kHz for 4 h. This simple and economical method can be used for large scale production. Electron microscopic, spectroscopic and thermo gravimetric analysis techniques were used to characterize these carbon nanoparticles. Functionalized CNPs were further conjugated with anticancer drug-methotrexate and used as fluorescent nano-carriers. In this research work, we determined the in vitro bioactivity of CNPs-methotrexate conjugates by lactate dehydrogenase assay, cell adhesion assay and sulforhodamine B assay in human lung carcinoma cell line (H157). The CNPs showed promising biocompatibility and CNPs-MTX conjugates demonstrated potent cytotoxic effects and high anticancer activities in human lung cancer cell line.

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Keywords
CNPs, carbon nanoparticles; CNPs–MTX, carbon nanoparticles–methotrexate conjugates; EDC, 1-Ethyl-3-[3-dimethylaminopropyl] carbodiimide.HCl; EDS, Energy dispersive X-ray spectroscopy; EN, ethylene diamine; FBS, fetal bovine serum; HBSS, Hank's balanced sa
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Synthesis, characterization and in vitro evaluation of methotrexate conjugated fluorescent carbon nanoparticles as drug delivery system for human lung cancer targeting
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 153, December 2015, Pages 111–120
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us