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Probing the interaction of troxerutin with transfer RNA by spectroscopic and molecular modeling

Paper ID Volume ID Publish Year Pages File Format Full-Text
30114 44460 2015 8 PDF Available
Title
Probing the interaction of troxerutin with transfer RNA by spectroscopic and molecular modeling
Abstract

•We investigated the troxerutin, an antioxidant interaction with tRNA.•External binding with low binding constant values as compared to strong binders•Troxerutin may have potential therapeutic application.

The studies on the interaction between tRNA (transfer RNA) and small molecules are an area of remarkable recent attention. For this notion a fundamental knowledge of the molecular features involving the interaction of small molecules with tRNA is crucial. Hence, in the present study we have investigated the interaction of TXER (troxerutin), natural bioflavonoid rutin derivative with yeast tRNA by using various spectroscopic techniques and molecular docking studies. The UV absorption and fluorescence emission studies demonstrated external binding of TXER on tRNA with low binding constant values as compared to strong binders. Circular dichroism (CD) spectroscopy study revealed that TXER did not show any significant modification on native conformation of tRNA. Furthermore in electrochemical study, the complex of TXER–tRNA did not expose any noticeable positive potential peak shift which indicated an interaction of TXER with tRNA by electrostatic or external binding mode. The docking study showed that the hydrogen and hydrophobic interactions were involved in binding of TXER–tRNA with docking score − 7.0 kcal/mol. These findings led us to confirm the interaction of TXER on tRNA through external binding with low binding affinity, indicating its potential bioapplication in the future.

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Keywords
TransferRNA; Troxerutin; Interaction; Spectroscopy; Molecular docking
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Probing the interaction of troxerutin with transfer RNA by spectroscopic and molecular modeling
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 153, December 2015, Pages 137–144
Authors
, , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us