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Spent coffee ground extract suppresses ultraviolet B-induced photoaging in hairless mice

Paper ID Volume ID Publish Year Pages File Format Full-Text
30117 44460 2015 9 PDF Available
Title
Spent coffee ground extract suppresses ultraviolet B-induced photoaging in hairless mice
Abstract

•ESCG significantly inhibits UVB-induced ROS production in HaCaT cells.•ESCG effectively suppresses UVB-induced wrinkle formation in hairless mice.•ESCG significantly decreases epidermal thickness and water loss in hairless mice.•ESCG decreases UVB-induced gene expressions of MMP2 and MMP9.•ESCG protects collagen-1 from UVB-irradiation.

The aim of this study is to evaluate the effect of spent coffee ground (SCG) ethanol extract on UVB-induced skin aging in hairless mice. An ethanol extract of SCG (ESCG) was prepared using the residue remaining after extraction of oil from roasted SCG. High performance liquid chromatography (HPLC) analysis showed that the content of caffeine (41.58 ± 0.54 μg/mg) was higher than that of chlorogenic acid isomers (~ 9.17 μg/mg) in ESCG. ESCG significantly decreased the UVB-induced intracellular reactive oxygen species in HaCaT cells. UVB-induced wrinkle formation in mice dorsal skin was effectively reduced by ESCG administration; high dose of ESCG (5 g/L) caused the reduction of wrinkle area by 30% compared with UVB-treated control (UVBC). This result correlated with the ESCG-mediated decrease in epidermis thickness (25%). In addition, ESCG administration significantly reduced transdermal water loss (20%) and erythema formation (35%) derived from UVB exposure. Collagen type I (COL-1) level in dorsal skin was effectively recovered by ESCG administration. These results were supported by down-regulation of collagen-degrading matrix metalloproteinase 2 (MMP2) and 9 (MMP9) expressions. Our results indicate that ESCG protects mouse skin from UVB-induced photoaging by suppressing the expression of matrix metalloproteinases. Our study suggests that ESCG may be anti-photoaging agent.

Graphical abstractSpent Coffee Ground and its effect on wrinkle formation of dorsal skin in hairless mice. Spent Coffee Grounds (SCG, down). The daily administration of hairless mice with ESCG for 12 weeks was accompanied with exposure to UVB radiation three times each week. Hairless mouse dorsal skins were photographed, and replicas made from the back skin using a Replica full kit. The wrinkle area was determined using the Image J program. NC: Normal Control; UVBC: UVB Control.Figure optionsDownload full-size imageDownload as PowerPoint slide

Keywords
Spent coffee ground; UVB-induced photoaging; ROS scavenging activity; HaCaT cell; Hairless mouse
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Spent coffee ground extract suppresses ultraviolet B-induced photoaging in hairless mice
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 153, December 2015, Pages 164–172
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us