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Formulation and stabilization of riboflavin in liposomal preparations

Paper ID Volume ID Publish Year Pages File Format Full-Text
30141 44460 2015 9 PDF Available
Title
Formulation and stabilization of riboflavin in liposomal preparations
Abstract

•Kinetics of riboflavin (RF) photolysis on entrapment in liposomes has been evaluated.•There is a correlation between rate constants and concentration of phospholipid.•There is a correlation between %T of liposomes and phosphatidylcholine concentrations.•RF fluorescence quenching is a function of phospholipid content in liposomes.•Mechanism of photoreductive stabilization of RF in liposomes has been proposed.

A study of the formulation of liposomal preparations of riboflavin (RF) with compositional variations in the content of phosphatidylcholine (PC) and their entrapment efficiency (26–42%) have been conducted. Light transmission characteristics of the liposomal preparations have been determined to evaluate their effect on the amount of light passing through the system to initiate a photochemical reaction. Dynamic light scattering (DLS) and atomic force microscopy (AFM) have been used to study the physical characteristics of liposomes. The liposomal preparations of RF have been subjected to photolysis using visible light and the apparent first- order rate constant, kobs, for the degradation of RF have been determined. The values of kobs (1.73–2.29 × 10− 3 min− 1) have been found to decrease linearly with an increase in PC concentration in the range of 12.15 to 14.85 mM. Thus, an increase in PC concentration of liposomes leads to an increase in the stability of RF. RF and its main photoproduct, lumichrome (LC), formed in liposomes have been assayed by a two-component spectrometric method at 356 and 445 nm using an irrelevant absorption correction to compensate for the interference of liposomal components. The fluorescence measurements of RF in liposomes indicate excited singlet state quenching and the formation of a charge-transfer complex between RF and PC. It results in electron transfer from PC to RF to cause photoreduction and stabilization of RF.

Graphical abstractA plot of kobs for photolysis of riboflavin versus phosphatidylcholine concentration in liposomes showing a linear correlation with an increase in concentration.Figure optionsDownload full-size imageDownload as PowerPoint slide

Keywords
Liposomal formulation; Riboflavin; Kinetics; Photolysis; Stabilization; Fluorescence quenching
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Formulation and stabilization of riboflavin in liposomal preparations
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 153, December 2015, Pages 358–366
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us