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Chlorin e6-mediated photodynamic effect diminishes therapeutic potential of 5-aza-2′-deoxycytidine-based whole-tumour-cell vaccine in mice bearing squamous cell carcinoma SCCVII

Paper ID Volume ID Publish Year Pages File Format Full-Text
30153 44460 2015 8 PDF Available
Title
Chlorin e6-mediated photodynamic effect diminishes therapeutic potential of 5-aza-2′-deoxycytidine-based whole-tumour-cell vaccine in mice bearing squamous cell carcinoma SCCVII
Abstract

•5-AZA-CdR increases the expression of cancer–testis antigens in SCCVII cells.•This effect can be used for preparation of whole-tumour-cell vaccines.•Photodynamic effect diminished therapeutic potential of 5-AZA-CdR-based vaccine

After years of setbacks, therapeutic cancer vaccines have become an alternative treatment option. Among the diversity of targeted tumour associated antigens (TAA), cancer–testis antigens (CTAs) are promising targets for cancer immunotherapy because they are highly immunogenic; meanwhile, they are expressed in human tumours of different histological origin but not in adult somatic tissues. Epigenetic modifications, such as DNA methylation, regulate CTAs expression both in normal and cancer cells. 5-Aza-2'-deoxycytidine (5-AZA-CdR), a DNA hypomethylating drug, induces the expression of CTAs in neoplastic cells.In these studies, we used 5-AZA-CdR-mediated up-regulation of CTAs and chlorin e6-mediated photodynamic effect in the production of a whole-tumour-cell vaccine against murine squamous cell carcinoma SCCVII in C3H/HeN mice.The results show that 5-AZA-CdR can be used to elevate levels of diverse CTAs in SCCVII cells. The 5-AZA-CdR-based vaccine, combined with the systemic administration of 5-AZA-CdR, delayed tumour growth. However, the treatment had no effect on survival in mice, most likely because of the toxicity of systemic treatment with 5-AZA-CdR. The photodynamic effect diminished therapeutic potential of 5-AZA-CdR-based vaccine.Chemo-immunotherapy with 5-AZA-CdR and therapeutic cancer vaccines may be an alternative approach to cancer therapy. However, further studies are needed to optimize treatment and vaccine preparation protocols.

Keywords
5-AZA-CdR, 5-aza-2′-deoxycytidine; Ce6, chlorin e6; PDT, photodynamic therapy; PS, photosensitizer; SCCVII, squamous cell carcinomaCancer–testis antigens; DNA methylation; Cancer; Photodynamic therapy; Vaccine; Immunotherapy
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Chlorin e6-mediated photodynamic effect diminishes therapeutic potential of 5-aza-2′-deoxycytidine-based whole-tumour-cell vaccine in mice bearing squamous cell carcinoma SCCVII
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 153, December 2015, Pages 455–462
Authors
, , , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us