fulltext.study @t Gmail

Improvement of welan gum biosynthesis and transcriptional analysis of the genes responding to enhanced oxygen transfer by oxygen vectors in Sphingomonas sp.

Paper ID Volume ID Publish Year Pages File Format Full-Text
3016 147 2014 8 PDF Available
Title
Improvement of welan gum biosynthesis and transcriptional analysis of the genes responding to enhanced oxygen transfer by oxygen vectors in Sphingomonas sp.
Abstract

•Effectively enhancing oxygen transfer was studied in welan gum biosynthesis.•n-Hexadecane significantly improved welan gum yield of 33.9 ± 0.56 g/L.•Related genes expression levels were responded to high DO by oxygen vectors.•This work would be useful to industrial welan gum biosynthesis.

Oxygen transfer (kLa) is one of the most essential factors affecting microbial cell growth and metabolite formation. In this study, low-cost methods capable of effectively enhancing oxygen transfer were investigated by adding oxygen vectors into the fermentation broth. Upon addition of n-dodecane and n-hexadecane, the kLa of the fermentation medium reached remarkably higher values than that of the control. In response to the enhanced oxygen transfer, welan gum concentration and yield reached maxima of 33.9 ± 0.56 g/L and 0.761 ± 0.010 g/g, respectively. The DCW reached a maximum of 7.82 ± 0.18 g/L with n-dodecane addition. Welan gum productivity and yield were significantly affected by kLa under n-hexadecane addition because of the promotion of the highest transcriptional levels of genes in the metabolic flux for welan gum biosynthesis. In addition, transcription levels of TCA and electron transfer chain (ETC) genes exhibited maximum up-regulation under n-dodecane, which resulted in enhanced cell growth. These results suggest a mechanism for enhanced kLa using oxygen vectors to improve welan gum biosynthesis through regulation of transcriptional levels of ETC, TCA, and metabolic pathways for welan gum biosynthesis genes during fermentation.

Keywords
Welan gum; Biosynthesis; Dissolved oxygen; Oxygen transfer; Viscosity; Electron transfer chain
First Page Preview
Improvement of welan gum biosynthesis and transcriptional analysis of the genes responding to enhanced oxygen transfer by oxygen vectors in Sphingomonas sp.
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 90, 15 September 2014, Pages 264–271
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us