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Spectroscopic study on the interaction of celecoxib with human carbonic anhydrase II: Thermodynamic characterization of the binding process

Paper ID Volume ID Publish Year Pages File Format Full-Text
30164 44461 2009 8 PDF Available
Title
Spectroscopic study on the interaction of celecoxib with human carbonic anhydrase II: Thermodynamic characterization of the binding process
Abstract

The effect of celecoxib, a sulfonamide drug, on the structure and function of human carbonic anhydrase II (hCA II) was investigated by various spectroscopic techniques such as UV–Vis, fluorescence and circular dichroism (CD) spectroscopy and differential scanning calorimetry (DSC), in 20 mM Tris, pH 7.75 at 27 °C. Kinetic results revealed that celecoxib inhibits the esterase activity of hCA II in a linear competitive manner with Ki = 61.61 ± 3.05 nM. DSC data indicated that the thermal stability of the enzyme has a minor increment in the presence of celecoxib. Fluorescence measurements showed that the celecoxib acts as a quencher of the enzyme fluorescence and calculation of the protein surface hydrophobicity (PSH), using 1-anilinonaphthalene-8-sulfonate (ANS), revealed the decrement of its PSH upon interaction with the drug. Acrylamide quenching experiments indicated the less accessibility of the tryptophan residues to the acrylamide due to the presence of celecoxib. Stern–Volmer analysis of quenching data at different temperatures elucidated that the quenching of intrinsic fluorescence of hCA II is occurred through a static quenching mechanism. Analysis of the thermodynamic parameters of binding showed that hydrogen bonding and hydrophobic interactions play the major role in stabilization of the enzyme–drug complex. The Job’s plot confirmed the existence of one binding site for celecoxib in hCA II. The far- and near-UV CD experiments indicated that celecoxib causes a little increment in α-helicity content of hCA II whereas its flexibility is decreased somewhat upon celecoxib binding.

Keywords
Human carbonic anhydrase II; Celecoxib; Competitive inhibition; Fluorescence quenching; Binding study; Protein surface hydrophobicity
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Spectroscopic study on the interaction of celecoxib with human carbonic anhydrase II: Thermodynamic characterization of the binding process
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 97, Issue 3, 2 December 2009, Pages 161–168
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us