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Reactive oxygen species production in mitochondria of human gingival fibroblast induced by blue light irradiation

Paper ID Volume ID Publish Year Pages File Format Full-Text
30178 44463 2013 5 PDF Available
Title
Reactive oxygen species production in mitochondria of human gingival fibroblast induced by blue light irradiation
Abstract

•Blue light irradiation decreased the cell growth of gingival fibroblasts.•The cytotoxic effect of blue-light irradiation was observed in mitochondria.•Reactive oxygen species were induced in mitochondria by blue light irradiation.

In recent years, it has become well known that the production of reactive oxygen species (ROS) induced by blue-light irradiation causes adverse effects of photo-aging, such as age-related macular degeneration of the retina. Thus, orange-tinted glasses are used to protect the retina during dental treatment involving blue-light irradiation (e.g., dental resin restorations or tooth bleaching treatments). However, there are few studies examining the effects of blue-light irradiation on oral tissue. For the first time, we report that blue-light irradiation by quartz tungsten halogen lamp (QTH) or light-emitting diode (LED) decreased cell proliferation activity of human gingival fibroblasts (HGFs) in a time-dependent manner (<5 min). Additionally, in a morphological study, the cytotoxic effect was observed in the cell organelles, especially the mitochondria. Furthermore, ROS generation induced by the blue-light irradiation was detected in mitochondria of HGFs using fluorimetry. In all analyses, the cytotoxicity was significantly higher after LED irradiation compared with cytotoxicity after QTH irradiation. These results suggest that blue light irradiation, especially by LED light sources used in dental aesthetic treatment, might have adverse effects on human gingival tissue. Hence, this necessitates the development of new dental aesthetic treatment methods and/or techniques to protect HGFs from blue light irradiation during dental therapy.

Keywords
Blue light; Light-emitting diode; Quartz tungsten halogen; Reactive oxygen species; Human gingival fibroblast; Mitochondria
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Reactive oxygen species production in mitochondria of human gingival fibroblast induced by blue light irradiation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 129, 5 December 2013, Pages 1–5
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us