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Synthesis and biological studies of steroidal pyran based derivatives

Paper ID Volume ID Publish Year Pages File Format Full-Text
30182 44463 2013 12 PDF Available
Title
Synthesis and biological studies of steroidal pyran based derivatives
Abstract

•Steroid-based cancer chemotherapeutics of the type 4H-pyrans have been synthesized and characterized.•DNA binding studies were carried out by gel electrophoresis, UV–vis and fluorescence spectroscopy and molecular docking.•The steroidal pyrans cleave supercoiled pBR322 DNA via hydrolytic pathway.•MTT assay confirmed that compound showed potential cytotoxicity against different cancer cells.•Comet assay showed that compounds are involved in apoptotic degradation of DNA.

Steroid based cancer chemotherapeutic agents of the type 2′-amino-3′-cyanocholest-6-eno[5,7-de]4H-pyrans (1c–3c) have been synthesized and characterized by the various spectroscopic and analytical techniques. The DNA binding studies of compounds (1c–3c) with CT DNA were carried out by UV–vis and fluorescence spectroscopy and gel electrophoresis. The compounds (1c–3c) bind to DNA preferentially through electrostatic and hydrophobic interactions with Kb values found to be 5.4 × 103, 2.3 × 103 M−1 and 1.97 × 103 M−1, respectively indicating the higher binding affinity of compound (1c) towards DNA. The molecular docking study suggested that the electrostatic interaction of compounds (1c–3c) in between the nucleotide base pairs is due to the presence of pyran moiety in steroid molecule. All the compounds (1c–3c) cleave supercoiled pBR322 DNA via hydrolytic pathway, as validated by T4 DNA ligase assay. The compounds (1c–3c) were screened for in vitro cytotoxicity against the cancer and non-cancer cells SW480, A549, HepG2, HeLa, MCF-7, HL-60, DU-145, NL-20, HPC and HPLF by MTT assay. The compounds (1c–3c) were tested for genotoxicity (comet assay) involving apoptotic degradation of DNA and was analyzed by agarose gel electrophoresis and visualized by ethidium bromide staining. The results revealed that compound (1c) has better prospectus to act as cancer chemotherapeutic candidate which warrants further in vivo anticancer investigations.

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Keywords
4H-Pyran; DNA binding; Gel electrophoresis; MTT assay; Comet assay
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 129, 5 December 2013, Pages 36–47
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us