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New fluorogenic dyes for analysis of cellular processes by flow cytometry and confocal microscopy

Paper ID Volume ID Publish Year Pages File Format Full-Text
30192 44463 2013 10 PDF Available
Title
New fluorogenic dyes for analysis of cellular processes by flow cytometry and confocal microscopy
Abstract

•Spectral properties of unsymmetrical monomethine cyanine dyes, containing quinazolinium moiety have been investigated.•The ability of dyes to stain live or apoptotic cells were examined by flow cytometry and confocal laser scanning microscopy.•The dyes are suitable for analysis of cell viability, apoptosis and cell cycle at low concentrations.

Fluorescent microscopy and fluorescent imaging by flow cytometry are two of the fastest growing areas in the medical and biological research. Innovations in fluorescent chemistry and synthesis of new dye probes are closely related to the development of service equipment such as light sources, and detection techniques. Among compounds known as fluorescent labels, the cyanine-based dyes have become widely used since they have high excitation coefficients, narrow emission bands and high fluorescence upon binding to nucleic acids.The key methods for evaluation of apoptosis and cell cycle allow measuring DNA content by several flow cytometric techniques.We have synthesized new monomethine cyanine dyes and have characterized their applicability for staining of live and/or apoptotic cells. Imaging experiments by flow cytometry and confocal laser scanning microscopy (CLSM) have been also performed. Two of the dyes have shown high-affinity binding to the nuclei at high dilutions, up to 10−9 M. Flow cytometry and CLSM have confirmed that these dyes labeled selectively non-living, e.g. ethanol-fixed cells that makes them appropriate for estimations of cell viability and apoptosis. The novel structures proved to be appropriate also for analysis of the cell cycle.

Keywords
Cyanine dyes; DNA-binding dyes; Apoptosis; Flow cytometry; Fluorescence
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New fluorogenic dyes for analysis of cellular processes by flow cytometry and confocal microscopy
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 129, 5 December 2013, Pages 125–134
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us