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Imaging of fast chlorophyll fluorescence induction curve (OJIP) parameters, applied in a screening study with wild barley (Hordeum spontaneum) genotypes under heat stress

Paper ID Volume ID Publish Year Pages File Format Full-Text
30228 44465 2015 8 PDF Available
Title
Imaging of fast chlorophyll fluorescence induction curve (OJIP) parameters, applied in a screening study with wild barley (Hordeum spontaneum) genotypes under heat stress
Abstract

•Fast chlorophyll fluorescence induction curves were imaged.•JIP-Test parameters were recorded for different wild barley genotypes.•A Heat Resistance Index, based on the Performance Index, was introduced.•The tested genotypes differed in their heat resistance.•Spatial heterogeneities of JIP-Test parameters were observed after heat stress.

We quantified the influence of heat stress (HS) on PSII by imaging of parameters of the fast chlorophyll fluorescence (CF) induction (OJIP) kinetic of 20 genotypes of wild barley (Hordeum spontaneum) covering a broad geographical spectrum. We developed a standardised screening procedure, allowing a repetitive fluorescence measurement of leaf segments. The impact of HS was quantified by calculating a Heat Resistance Index (HRI), derived from the decrease of the Performance Index (PI) caused by HS treatment and following recovery. For the genotype showing the lowest HRI, reduced maximum quantum yield (φP0) and increased relative variable fluorescence of the O-J phase (K-Peak) were detected after HS, whereas the basal fluorescence (F0) remained stable. An additional feature was a lowered fraction of active (QA-reducing) reaction centres (RCs). The disturbances disappeared after one day of recovery. Spatial heterogeneities of fluorescence parameters were detected, as the negative effect of HS was stronger in the leaf areas close to the leaf tip. The results of this study prove that chlorophyll fluorescence imaging (CFI) is suitable for the detection of HS symptoms and that imaging of JIP-Test parameters should be considered in future screening and phenotyping studies aiming for the characterisation of plant genotypes.

Keywords
CCD, charge-coupled device; CF, chlorophyll fluorescence; CFI, chlorophyll fluorescence imaging; DF, driving force; FV/FM, maximum quantum efficiency of PSII; HRI, Heat Resistance Index; HS, heat stress; NPQ, nonphotochemical quenching; OEC, oxygen evolvi
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Imaging of fast chlorophyll fluorescence induction curve (OJIP) parameters, applied in a screening study with wild barley (Hordeum spontaneum) genotypes under heat stress
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 151, October 2015, Pages 153–160
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us