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The effects of combined low level laser therapy and mesenchymal stem cells on bone regeneration in rabbit calvarial defects ☆

Paper ID Volume ID Publish Year Pages File Format Full-Text
30231 44465 2015 6 PDF Available
Title
The effects of combined low level laser therapy and mesenchymal stem cells on bone regeneration in rabbit calvarial defects ☆
Abstract

•Low level laser therapy increased new bone generation in calvarial bone defects.•Combination of MSCs and LLLT did not increase bone regeneration in 3 weeks.•Low level laser therapy decreased inflammation of bone defects.

ObjectiveThis study evaluated the effect of Low Level Laser Therapy (LLLT) and Mesenchymal Stem Cells (MSCs) on bone regeneration.Background dataAlthough several studies evaluated the effects of MSCs and LLLT, there is little information available regarding in vivo application of LLLT in conjunction with MSCs.MethodsForty-eight circular bone defects (6 mm in diameter) were prepared in the calvaria of 12 New-Zealand white rabbits. The defects of each animal were randomly assigned to 4 groups: (C) no treatment; (L) applying LLLT; (SC) filled with MSCs; (SCL) application of both MSCs and LLLT. LLL was applied on alternate days at wavelength of 810 nm, power density of 0.2 W/cm2 and a fluency of 4 J/cm2 using a Gallium–Aluminum–Arsenide (GaAlAs) diode laser. The animals were sacrificed after 3 weeks and then histological samples were evaluated to determine the amount of new bone formation and the remaining scaffold and inflammation.ResultsThe histological evaluation showed a statistically significant increase in new bone formation of LLLT group relative to the control and the other two experimental groups (p < 0.05). There was no significant difference in bone formation of the control group compared to experimental groups filled with MSCs. Laser irradiation had no significant effect on resorption of the scaffold material. In addition, inflammation was significantly reduced in LLLT group compared to the control defects and the other two experimental groups.ConclusionLow level laser therapy could be effective in bone regeneration but there is no evidence of a synergistic effect when applied in conjunction with MSCs.

Keywords
Low Level Laser Therapy (LLLT); Bone regeneration; Stem cell; Animal study
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The effects of combined low level laser therapy and mesenchymal stem cells on bone regeneration in rabbit calvarial defects ☆
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 151, October 2015, Pages 180–185
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us