fulltext.study @t Gmail

In vitro DNA binding studies of antiretroviral drug nelfinavir using ethidium bromide as fluorescence probe

Paper ID Volume ID Publish Year Pages File Format Full-Text
30233 44465 2015 7 PDF Available
Title
In vitro DNA binding studies of antiretroviral drug nelfinavir using ethidium bromide as fluorescence probe
Abstract

•NFV bind to minor groove region of hs DNA.•Static quenching mechanism involve in binding between NFV and hs DNA.•Hydrogen bonding played major role in binding.

Understanding the interaction of small molecules with DNA has become an active research area at the interface between biology and chemistry. In the present work, we investigated the mode of interaction of nelfinavir (NFV) with herring sperm DNA (hs DNA) under physiological conditions using various biophysical techniques. Analysis of UV-absorption and fluorescence spectra indicates the formation of complex between NFV and hs DNA. According to the fluorescence results, the binding constant (K) between NFV and hs DNA was found to be 3.30 × 104 LM−1. The calculated thermodynamic parameters (ΔH° and ΔS°) suggested that hydrogen bonding plays a major role in binding between them. Phosphate group binding studies revealed that there was no electrostatic interactions occurred between NFV and hs DNA. Circular dichroism (CD) and DNA melting curve were employed to measure the conformational change of hs DNA in the presence of NFV, which verified the minor groove binding mode. These results were further supported by viscosity measurements and competitive displacement assay study using Hoechst 33258. According to the sequence specificity experiments, NFV binds to A–T rich region of hs DNA.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slide

Keywords
Nelfinavir; Hs DNA; Minor groove binding; Hydrogen bonding
First Page Preview
In vitro DNA binding studies of antiretroviral drug nelfinavir using ethidium bromide as fluorescence probe
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 151, October 2015, Pages 194–200
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us