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Methylamine interaction with proteins of photosystem II: A comparison with biogenic polyamines

Paper ID Volume ID Publish Year Pages File Format Full-Text
30253 44466 2009 6 PDF Available
Title
Methylamine interaction with proteins of photosystem II: A comparison with biogenic polyamines
Abstract

Biogenic polyamines are essential for cell growth and differentiation. The interaction of polyamines with protein of photosystem II (PSII) are well investigated, while there has been no report on the effect of monoamines complexation on photosynthetic oxygen evolution. This study was designed to investigate the interaction of methylamine with proteins of PSII, using PSII-enriched submembrane fractions with various concentrations of methylamine. Fourier transformed infrared (FTIR) and fluorescence spectroscopic methods were used in order to determine the methylamine binding mode, the protein conformational changes, and the effect of amine interaction on photosynthetic oxygen evolution. Spectroscopic evidence showed that methylamine interacts with protein (H-bonding) through polypeptide CO, C–N and NH groups with major perturbations of protein secondary structure. Major reduction of α-helix from 50% (free PSII) to 35% with an increase of β-sheet from 10% (free PSII) to 16% was observed in methylamine-PSII complexes. At very low methylamine concentration, no inhibition of oxygen-evolution occurred, while at higher amine content (12 mM), 100% inhibition was observed. Chlorophyll (Chl) fluorescence measurements indicated the inhibition mainly affects the oxygen evolving complex (OEC) of PSII. Comparisons of the effects of methylamine with biogenic polyamine spermine, spermidine and putrescine showed a similar mode of binding with protein (H-bonding) through polypeptide CO, C–N and NH groups. However, major alterations of the protein secondary structure are induced by monoamine and not by polyamines.

Keywords
Methylamine; Photosystem II; Oxygen-evolution; Protein secondary structure; FTIR spectroscopy
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Methylamine interaction with proteins of photosystem II: A comparison with biogenic polyamines
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 96, Issue 3, 4 September 2009, Pages 201–206
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us