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FTIR of touch imprint cytology: A novel tissue diagnostic technique

Paper ID Volume ID Publish Year Pages File Format Full-Text
30268 44467 2008 5 PDF Available
Title
FTIR of touch imprint cytology: A novel tissue diagnostic technique
Abstract

Fourier transform infrared spectroscopic (FTIR) interrogation of biological tissues in real time has largely been a challenging proposition because of the strong absorption of mid-infrared light in water filled tissues. To enable sampling of tissues they must be sectioned and dried, which has time and resource implications. FTIR of touch imprint cytology (TIC) has been proposed to circumvent this problem. TIC is a well known histopathological method of rapidly analysing biological tissues. In this article we demonstrate the ability of FTIR of TIC to provide detailed spectra which can be used to differentiate various tissue pathologies. FTIR spectral profiles of TIC of lymph node and thyroid tissues differ visually when compared with TIC spectra of parathyroid tissue. The lymph node showed strong lipid spectral peaks at 1166 cm−1 and 1380 cm−1 including a very strong carbonyl-ester band at 1748 cm−1, and a strong methylene bending band (scissoring, at 1464 cm−1). Smaller intensity protein peaks at 1547 cm−1 and 1659 cm−1 were also seen. The thyroid spectra, in addition to evident strong protein peaks at 1547 cm−1 and 1659 cm−1, also demonstrated possible nucleic acid signals at 1079 cm−1 and 1244 cm−1. The C-OH peak at 1037 cm−1 was attributed to carbohydrate signals. Parathyroid adenoma showed a marginal shift to lower wavenumbers with decreased amide I and II peak intensities when compared to hyperplasia. Nucleic acid peak positions at 1079 cm−1 and 1244 cm−1 were of higher intensity in adenomas compared to hyperplastic glands possibly demonstrating an increase in cell proliferation and growth.This study demonstrates the feasibility of cytoimprint FTIR for the intraoperative diagnosis of tissue during surgical neck exploration for the management of hyperparathyroidism. There is potential for the application of the technique in sentinel lymph node biopsy diagnosis and tumour margin evaluation.

Keywords
Infrared spectroscopy; Diagnosis; Imprint cytology; Parathyroid; Thyroid; Lymph node
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 92, Issue 3, 18 September 2008, Pages 160–164
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us