fulltext.study @t Gmail

Spectroscopic study on the interaction between naphthalimide–polyamine conjugates and DNA

Paper ID Volume ID Publish Year Pages File Format Full-Text
30299 44468 2014 9 PDF Available
Title
Spectroscopic study on the interaction between naphthalimide–polyamine conjugates and DNA
Abstract

•Polyamine conjugates caused the conformational alteration of DNA.•Naphthalimide–polyamine conjugates intercalated into herring sperm DNA.•The binding strength was associated with substituted groups on naphthalene backbone.•The fluorescent quenching mechanism was a static type.•The type of interaction force was mainly hydrogen bonding.

The interaction of naphthalimide–polyamine conjugates with herring sperm DNA was studied by UV/vis absorption and fluorescent spectra under physiological conditions (pH = 7.4). The observed spectral quenching of compounds by DNA and the displacement of EB from DNA-EB complex by compounds indicated that these naphthalimide–polyamine conjugates could intercalate into the DNA base pairs. The UV test also showed that these compounds caused the conformational alteration of DNA. Further caloric fluorescent tests revealed that the quenching mechanism was a static type, which Ksv of 1-DNA, 2-DNA and 1-DNA-EB, 2-DNA-EB 3-DNA-EB was 1.208 × 104, 7.792 × 103 and 1.712 × 104, 1.287 × 104, 2.874 × 104, respectively, at room temperature. The obtained quenching constant, binding constant and thermodynamic parameters suggested that binding strength was associated with substituted groups on naphthalene backbone, and the type of interaction force included mainly hydrogen bonding and weak van der Waals. The binding process was mainly driven by hydrogen bond and van der Waals. Additionally, the effect of NaCl on compounds-DNA interaction provided further evidence that their interaction modes were dependent on substituted groups.

Graphical abstractThe interaction between naphthalimide–polyamine conjugates with herring sperm DNA was studied by UV/vis absorption and fluorescent spectra under physiological conditions.Figure optionsDownload full-size imageDownload as PowerPoint slide

Keywords
Naphthalimides; Polyamine conjugates; DNA; Spectroscopic methods
First Page Preview
Spectroscopic study on the interaction between naphthalimide–polyamine conjugates and DNA
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 138, 5 September 2014, Pages 202–210
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us