fulltext.study @t Gmail

Location and function of the high-affinity chloride in the oxygen-evolving complex – Implications from comparing studies on Cl−/Br−/I−-substituted photosystem II prepared using two different methods

Paper ID Volume ID Publish Year Pages File Format Full-Text
30304 44468 2014 7 PDF Available
Title
Location and function of the high-affinity chloride in the oxygen-evolving complex – Implications from comparing studies on Cl−/Br−/I−-substituted photosystem II prepared using two different methods
Abstract

•Two Types of Cl−/Br−/I− substituted PSII have been prepared and investigated.•Remarkable changes of OEC are observed only in Type 2 preparations.•The high-affinity Cl− plays key roles for water oxidation in PSII.•The location of high-affinity Cl− may be different to those revealed in X-ray data.

The high-affinity chloride ion (Cl−) is known to play a key role in water oxidation in photosystem II (PSII). Recent crystallographic studies revealed two Cl− binding sites in PSII. To examine whether these two Cl− ions are correlated to the high-affinity Cl−, we prepared Cl−/Br−/I−-substituted PSII samples from both higher plants and cyanobacteria by using two different protocols: one was the method used in the crystallographic study (Type 1) and the other was a method developed recently to ensure the efficient replacement of Cl− (Type 2). While only minor effects were observed in the Type 1 preparation, efficient Br−/I−substitution by the Type 2 protocol led to significant changes in the EPR properties of the oxygen-evolving complex (OEC) and the TyrZ, as well as in oxygen-evolving activities. These results are discussed in terms of the binding site of the high-affinity Cl− relative to the two Cl− ions revealed by the recent X-ray structural data.

Graphical abstractThe high-affinity Cl− is known to play roles for function of PSII. Two types of Cl−/Br−/I− substituted PSII samples were prepared to probe its location and function. Significant changes of the OEC was observed only in preparations with efficient Br−/I−substitution, but not in the method used in recent crystallographic study. These results provide new insights to understanding the function and location of the high-affinity Cl− in PSII.Figure optionsDownload full-size imageDownload as PowerPoint slide

Keywords
Photosystem II; Oxygen-evolving complex; High-affinity chloride; Br−/I− substitution; Electron paramagnetic resonance
First Page Preview
Location and function of the high-affinity chloride in the oxygen-evolving complex – Implications from comparing studies on Cl−/Br−/I−-substituted photosystem II prepared using two different methods
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 138, 5 September 2014, Pages 249–255
Authors
, , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us