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Improved endoxylanase production and colony morphology of Aspergillus niger DSM 26641 by γ-ray induced mutagenesis

Paper ID Volume ID Publish Year Pages File Format Full-Text
3031 148 2015 6 PDF Available
Title
Improved endoxylanase production and colony morphology of Aspergillus niger DSM 26641 by γ-ray induced mutagenesis
Abstract

•β-1,4-Endoxylanase activity of Aspergillus niger DSM 26641 was doubled by γ-radiation.•Restricted widespread filamentous growth on agar plates was observed.•Miniaturization and pellet surface restriction favors biomass recycling.

Aspergillus niger DSM 26641 was exposed to 60Co γ-radiation to enhance the β-1,4-endoxylanase activity, restrict colony growth and improve robustness of pellets. The first promising mutant obtained after γ-radiation of the fungal spores at 50–2000 Gy showed a restricted colony growth and an 82% enhancement in β-1,4-endoxylanase activity. The mutant was subjected to a second round of γ-radiation at 1400 Gy generating a mutant with double the β-1,4-endoxylanase activity compared to the native strain. The selected final mutant, deposited as A. niger DSM 28712, showed a maximal saccharification activity of 26 U ml−1 on xylan based broth, 48 U ml−1 on lignocellulose hydrolysate and 375 U ml−1 on lignocellulose hydrolysate supplemented with yeast extract and mineral salts.

Keywords
Filamentous fungi; γ-radiation mutagenesis; Enzymes; Endoxylanase; Microbial growth; Optimization
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Improved endoxylanase production and colony morphology of Aspergillus niger DSM 26641 by γ-ray induced mutagenesis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 94, 15 February 2015, Pages 9–14
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us