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Luminol-based bioluminescence imaging of mouse mammary tumors

Paper ID Volume ID Publish Year Pages File Format Full-Text
30342 44469 2013 6 PDF Available
Title
Luminol-based bioluminescence imaging of mouse mammary tumors
Abstract

•Luminol can be used to track neutrophil invasion of tumors.•For the first time, we have shown Luminol can be used to image solid carcinomas in mice.•Luminol imaging resulted in detection of tumors before they were palpable.

Polymorphonuclear neutrophils (PMNs) are the most abundant circulating blood leukocytes. They are part of the innate immune system and provide a first line of defense by migrating toward areas of inflammation in response to chemical signals released from the site. Some solid tumors, such as breast cancer, also cause recruitment and activation of PMNs and release of myeloperoxidase. In this study, we demonstrate that administration of luminol to mice that have been transplanted with 4T1 mammary tumor cells permits the detection of myeloperoxidase activity, and consequently, the location of the tumor. Luminol allowed detection of activated PMNs only two days after cancer cell transplantation, even though tumors were not yet palpable. In conclusion, luminol-bioluminescence imaging (BLI) can provide a pathway towards detection of solid tumors at an early stage in preclinical tumor models.

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Keywords
Bioluminescence; Chemiluminescence; Neutrophil; Luminol; Myeloperoxidase (MPO); Cancer detection
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 127, 5 October 2013, Pages 223–228
Authors
, , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us