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Effect of preillumination with red light on photosynthetic parameters and oxidant-/antioxidant balance in Arabidopsis thaliana in response to UV-A

Paper ID Volume ID Publish Year Pages File Format Full-Text
30343 44469 2013 8 PDF Available
Title
Effect of preillumination with red light on photosynthetic parameters and oxidant-/antioxidant balance in Arabidopsis thaliana in response to UV-A
Abstract

•We studied the effect of preillumination with red light on photosynthetic parameters.•Oxidant-/antioxidant balance in Arabidopsis thaliana.•Response to UV-A.

The effect of preillumination with low intensity (10 μmol quanta m−2 s−1, 10 min) light of different wavelengths in the spectral range of 550–730 nm on photosynthesis and activity of PSII, the content of photosynthetic pigments and H2O2, as well as the peroxidase activity in the leaves of 26-d-old Arabidopsis thaliana wild-type (WT) plants in response to UV-A radiation was studied. UV-A decreased the activity of the PSII, the content of Chl a, Chl b and carotenoids, as well as increased the peroxidase activity and H2O2 level in the WT leaves. Preillumination of the leaves with red light (RL, λmax = 664 nm) reduced the inhibitory effect of UV radiation on photosynthesis and activity of the PSII, indicated by delayed light emission as well as the H2O2 level, but increased the peroxidase activity in the leaves compared to illumination by UV radiation only. Illumination with RL alone and the subsequent exposure of plants to darkness increased the peroxidase activity and the transcription activity of genes of the transcription factors APX1 and HYH. Preillumination of leaves with RL, then far red light (FRL, λmax = 727 nm) partially compensated the effect of the RL for all studied parameters, suggesting that the active form of phytochrome (PFR) is involved in these processes. Preillumination with the wavelengths of 550, 594 and 727 nm only did not have a marked effect on photosynthesis. The hy2 mutant of Arabidopsis with reduced synthesis of the phytochrome B chromophore showed decreased resistance of PSII to UV-A compared with the WT of Arabidopsis. UV radiation reduced Chl a fluorescence much faster in the hy2 mutant compared to the WT. Preillumination of the hy2 mutant with RL did not affect the PSII activity and H2O2 level in UV-irradiated leaves. It is assumed that the formation of the increased resistance of the photosynthetic apparatus of Arabidopsis to UV-A radiation involves PFR and the antioxidant system of plants, partly by inducing transcriptional activity of some antioxidant and transcription factors genes.

Keywords
Arabidopsis thaliana; Red light illumination; H2O2; Stress-resistance; UV-A; Transcription
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Effect of preillumination with red light on photosynthetic parameters and oxidant-/antioxidant balance in Arabidopsis thaliana in response to UV-A
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 127, 5 October 2013, Pages 229–236
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us