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Alteration of photochemistry and protein degradation of photosystem II from Chlamydomonas reinhardtii under high salt grown cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
30361 44472 2013 8 PDF Available
Title
Alteration of photochemistry and protein degradation of photosystem II from Chlamydomonas reinhardtii under high salt grown cells
Abstract

•Effects of high salt on photochemistry and protein degradation of PSII in Chlamydomonas reinhardtii.•Energy transfer within PSII is impaired.•Pigment–pigment interactions were changed.•Differential degradation of both reaction and LHCII proteins.

In this study, we evaluated the inhibitory effect of NaCl on cell growth, photochemistry and protein profile of photosystem (PS) II in Chlamydomonas reinhardtii. To study the effect of NaCl on the photosynthetic apparatus, the C. reinhardtii cells were grown at different concentrations (0, 50, 100 and 150 mM). NaCl induced flagellar resorption due to which the cells lost their motility, formation of palmelloids, reduced cell size and slower cell division. Chlorophyll fluorescence transients at different NaCl concentrations had decreased intensities of all peaks (OJIP) indicating the apparent inactivation energies of both donor and acceptor side of PSII. Consequently, inhibition of electron transport occurred particularly at PSII. Further, low temperature emission spectra showed that the rate of damage to the PSII was more when compared to PSI. Also, we have carried out the visible circular dichroism spectra from thylakoids where the major peaks contributed to chlorophyll a and b are equally reduced in different salt grown cells, which may explain the changes at the level of inter pigment–pigment interactions. Furthermore protein profile analysis of PSII revealed that the major subunit of light harvesting complex (LHC)II is more prone to salt stress than core proteins of PSII indicating the light harvesting funnel from LHCII to PSII core is impaired.

Keywords
Chl fluorescence; Reaction centre proteins; LHCII proteins; Photosystems; Salt stress; Thylakoid membrane
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Alteration of photochemistry and protein degradation of photosystem II from Chlamydomonas reinhardtii under high salt grown cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 124, 5 July 2013, Pages 63–70
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us