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Ex vivo quantification of mTHPC concentration in tissue: Influence of chemical extraction on the optical properties

Paper ID Volume ID Publish Year Pages File Format Full-Text
30367 44474 2008 9 PDF Available
Title
Ex vivo quantification of mTHPC concentration in tissue: Influence of chemical extraction on the optical properties
Abstract

A method for the quantification of the concentration of the photosensitizer meso-tetra(hydroxyphenyl) chlorin (mTHPC) in tissue samples is presented. The technique is an extension of a previously published method based on alkaline hydrolysis of tissue, using Solvable™ as a tissue solubilizer. mTHPC quantification was achieved by subsequent fluorescence spectroscopy. Since the original extraction method involved multiple steps in which water dilution of the sample was implemented, we studied the spectral characteristics of mTHPC in different Solvable™/water mixtures. Using UV–VIS absorption and fluorescence spectroscopy, it was demonstrated that the spectral characteristics of mTHPC vary for different Solvable™ concentrations. In the range of 20–100% Solvable™, the fluorescence intensity of mTHPC did not change, while dramatic changes in the mTHPC fluorescence intensity were observed for lower Solvable™ concentrations (< 20%) due to increasing hydrophilicity of the environment, combined with pH alterations. We also demonstrated that the absorption and fluorescence spectra of the dissolved tissue were time-dependent. Longer incubation of the samples resulted in a significant increase of the native tissue chromophore fluorescence. This implies that for the correct quantification of photosensitizer concentrations, the fluorescence of native tissue chromophores must be accounted for.

Keywords
mTHPC; mTHPC quantification; Extraction method; Solvable™
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Ex vivo quantification of mTHPC concentration in tissue: Influence of chemical extraction on the optical properties
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 91, Issues 2–3, 29 May 2008, Pages 99–107
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
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