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Influence of glycerol on poly(3-hydroxybutyrate) production by Cupriavidus necator and Burkholderia sacchari

Paper ID Volume ID Publish Year Pages File Format Full-Text
3037 148 2015 8 PDF Available
Title
Influence of glycerol on poly(3-hydroxybutyrate) production by Cupriavidus necator and Burkholderia sacchari
Abstract

•LMW-PHB production by two collection strains using glycerol.•Glycerol differently influences growth kinetics, PHB production and its Mw.•Combining glycerol & glucose improve growth and PHB production by C. Necator.•Using glycerol as a sole carbon source by B. sacchari produces LMW-PHB.

Glycerol is a co-product of many industrial processes and is generated in large quantities from different origins. In this study, glycerol is used as a cheap carbon source for the production of poly(3-hydroxybutyrate) (PHB) with two different collection strains, Cupriavidus necator and Burkholderia sacchari, in order to provide an alternative outlet for glycerol and produce value-added bioproducts. The objective of this work was to study the influence of this carbon source on their growth kinetics, on their polymer production, and on the molecular mass of the produced biopolymer. Therefore, fermentations in bioreactors were carried out with these strains. Different results for both strains were obtained showing, for the first time, a high cell dry mass and growth rate, when glycerol was used together with glucose in the fermentation with C. necator. In the first fermentation with B. sacchari using glycerol as a sole carbon source, the strain properly developed synthesizing PHB. The biopolymers obtained from both fermentations with glycerol showed low molecular masses about 300 kDa with a polydispersity of 4.72 with C. necator, and 200 kDa with polydispersity of 2.50 with B. sacchari.

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Keywords
Cupriavidus necator; Burkholderia sacchari; Poly(3-hydroxybutyrate); Glycerol; Bioreactors; Molecular mass; Fed-Batch Culture; Fermentation; Microbial Growth
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Influence of glycerol on poly(3-hydroxybutyrate) production by Cupriavidus necator and Burkholderia sacchari
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 94, 15 February 2015, Pages 50–57
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us