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Spectroscopic analysis of the interaction between gallium(III) and apoovotransferrin

Paper ID Volume ID Publish Year Pages File Format Full-Text
30372 44474 2008 6 PDF Available
Title
Spectroscopic analysis of the interaction between gallium(III) and apoovotransferrin
Abstract

Ovotransferrin is a main member of transferrin family and has a dual role in both the transport of iron and antibacterial function. Gallium-67 is widely used as an imaging agent for tumors. It has been reported that Ga3+ can bind to apoovotransferrin at two sites, one in the N-terminal lobe and another in the C-terminal lobe. However, several details of the interaction between Ga3+ and apoOTf remain unclear. Here, we report detailed investigations into the interactions of Ga3+ with apoovotransferrin at the molecular level. First, the characteristics of Ga3+ binding to apoovotransferrin were analyzed using UV difference spectra. The results show that Ga3+ prefers to bind to the N-terminal site rather than the C-terminal site under the experimental conditions. Effective stability constants of log KN = 18.88 ± 0.24 and log KC = 17.65 ± 0.12 were determined. Second, conformational changes in apoovotransferrin during Ga3+ binding were studied using 2-p-toluidinylnaphthalene-6-sulfonate (TNS) as a fluorescence probe. Apoovotransferrin undergoes a large conformational change when Ga3+ binds to the N-terminal site, and a smaller conformational change when the ion binds to the C-terminal site. UV difference spectra were also used to measure the rate at which EDTA removes Ga3+ from ovotransferrin carrying one Ga3+ at the N-terminal site. Ga3+ removal from the N-terminal binding site follows simple saturation kinetics.

Keywords
Ovotransferrin; Ga3+; Spectra; Conformational change; Kinetics
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Spectroscopic analysis of the interaction between gallium(III) and apoovotransferrin
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 91, Issues 2–3, 29 May 2008, Pages 137–142
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us