fulltext.study @t Gmail

Effects of oxygen-vectors on the synthesis of epsilon-poly-lysine and the metabolic characterization of Streptomyces albulus PD-1

Paper ID Volume ID Publish Year Pages File Format Full-Text
3038 148 2015 7 PDF Available
Title
Effects of oxygen-vectors on the synthesis of epsilon-poly-lysine and the metabolic characterization of Streptomyces albulus PD-1
Abstract

•n-Dodecane exhibited a stimulatory effect on ɛ-PL production.•A novel strategy to improve ɛ-PL production was developed.•The metabolic pathway of ɛ-PL was enhanced by 0.5% n-dodecane.

The production of epsilon-poly-lysine (ɛ-PL) was enhanced by adding oxygen-vectors to the culture broth of Streptomyces albulus PD-1. ɛ-PL concentration reached a maximum of 30.8 ± 0.46 g/L and the dry cell weight was 33.8 ± 0.29 g/L when 0.5% n-dodecane was added to fed-batch fermentation. This improvement in ɛ-PL production and cell mass production can be related to 0.5% n-dodecane that could maintain dissolved oxygen concentration >32% saturation throughout the course of the ɛ-PL production phase. The transcription level of key genes and enzyme activities were analyzed. Results showed that the metabolic pathway responsible for ɛ-PL synthesis was strengthened when n-dodecane was added. The change in intracellular nucleotide levels was also investigated. Higher ATP level and a lower NADH/NAD+ ratio were obtained with 0.5% n-dodecane in broth, thereby providing more energy for ɛ-PL synthesis. These results indicated that ɛ-PL synthesis was strengthened from carbon metabolism and energy metabolism with 0.5% n-dodecane in broth.

Keywords
Biosynthesis; Dissolved oxygen; Fermentation; Polypeptides; Epsilon-poly-lysine; Oxygen-vectors.
First Page Preview
Effects of oxygen-vectors on the synthesis of epsilon-poly-lysine and the metabolic characterization of Streptomyces albulus PD-1
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 94, 15 February 2015, Pages 58–64
Authors
, , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us