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Nonadiabatic tapered optical fiber sensor for measurement of antimicrobial activity of silver nanoparticles against Escherichia coli

Paper ID Volume ID Publish Year Pages File Format Full-Text
30387 44475 2014 10 PDF Available
Title
Nonadiabatic tapered optical fiber sensor for measurement of antimicrobial activity of silver nanoparticles against Escherichia coli
Abstract

•Fabricate a label free optical fiber biosensor for monitoring antibacterial activity of silver nanoparticle (SNP).•Rapid analysis compared to conventional bacteriological techniques.•We examine the influence of the bacterial media on the antibacterial effectiveness of the SNPs.•We measure inhibition bacterial growth rate with colony counting and fiber optic biosensor.•We report a novel sensing method for study mechanism of antibacterial activity of SNPs with biosystems.

Silver nanoparticles (SNPs) exhibit antibacterial properties via bacterial inactivation and growth inhibition but the mechanism is not yet completely understood. In this study a label free and rapid detection method for study of antimicrobial activity of the SNP against Escherichia coli (E. coli K-12) is investigated using a nonadiabtic tapered fiber optic (NATOF) biosensor. The results show that SNPs interact with bacteria either by anchoring to or penetrating into the bacterial cell layer. These mechanism changes the refractive index (RI) of the tapered region, which in turn lead to the changes in the optical characteristics of the tapered fiber and output signals. With similar conditions for bacteria, the inhibition rate of the E. coli growth was measured by colony counting method as an experimental control and the results were compared with those obtained from the fiber sensor measurements. For SNP concentrations ranging from 0 to 50 μg ml−1 the inhibition rates of the E. coli growth were measured to be from 1.27 h−1 to −0.69 h−1 and from −3.00 × 10−3 h−1 to −1.98 × 10−2 h−1 for colony counting and optical fiber biosensor, respectively. The results demonstrate the potential of the proposed NATOF biosensor as a label free and rapid sensing platform for understanding the mechanism of antibacterial effects of SNPs.

Keywords
Silver nanoparticle; Nonadiabatic tapered optical fiber; E. coli; Refractive index; Biosensor and inhibition rate
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Nonadiabatic tapered optical fiber sensor for measurement of antimicrobial activity of silver nanoparticles against Escherichia coli
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 135, 5 June 2014, Pages 55–64
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us