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Interactions of peripheral-type benzodiazepine receptor with diamino acid derivatives of protoporphyrin IX

Paper ID Volume ID Publish Year Pages File Format Full-Text
30400 44476 2009 5 PDF Available
Title
Interactions of peripheral-type benzodiazepine receptor with diamino acid derivatives of protoporphyrin IX
Abstract

The aim of this study was to examine and determine binding affinities of the peripheral benzodiazepine receptor (PBR) with diamino acid derivatives of protoporphyrin IX with general structure PP(AA)2 in a function of environmental pH changes. Fluorescence intensity was used as a measure of protein association constant.Decrease in protein fluorescence, after titration with increasing concentration of porphyrins, gave evidence of interactions between PBR and examined ligands. Experimental data were fitted to theoretical curves, assuming different models of interactions. All examined ligands were best fitted to weak binding model.Increase in environmental acidity resulted in changes in association constants. For all examined derivatives association constants were at least twice higher in pH 5.5 as compared to pH 7.4 and 6.5.

Keywords
Peripheral benzodiazepine receptor; Porphyrin derivatives; Binding affinities
First Page Preview
Interactions of peripheral-type benzodiazepine receptor with diamino acid derivatives of protoporphyrin IX
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 94, Issue 2, 9 February 2009, Pages 138–142
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering