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Spectroscopic studies on the binding of bromocresol purple to different serum albumins and its bilirubin displacing action

Paper ID Volume ID Publish Year Pages File Format Full-Text
30403 44477 2008 7 PDF Available
Title
Spectroscopic studies on the binding of bromocresol purple to different serum albumins and its bilirubin displacing action
Abstract

The interaction between bromocresol purple (BCP) and bovine serum albumin (BSA)/porcine serum albumin (PSA) was investigated both in the absence and presence of bilirubin (BR) using absorption/absorption difference spectroscopy. A significant red shift in the absorption maxima of BCP accompanied by a decrease in absorbance was indicative of BCP binding to albumin. The titration of BSA and PSA with BCP using absorption difference spectroscopy and analysis of results by Benesi–Hildebrand equation yielded the values of association constant, K as 9.9 ± 0.9 × 104 L mol−1 and 4.1 ± 0.3 × 104 L mol−1 for BSA and PSA, respectively. The differential extinction coefficient (Δε) of 34,484 M−1 cm−1 at 615 nm and 41,870 M−1 cm−1 at 619 nm were estimated for BSA and PSA, respectively. Decrease in (ΔAbs.)615 nm of BCP–BSA complex with the increase in ionic strength suggested the role of hydrophobic interactions in the binding phenomenon. A significant blue shift in the absorption maxima and change in (ΔAbs.)λmax(ΔAbs.)λmax values of BR–albumin complexes upon addition of increasing concentrations of BCP revealed the BR displacing action of BCP on albumin molecule.

Keywords
Absorption difference spectra; Bilirubin displacement; Bovine serum albumin; Bromocresol purple; Porcine serum albumin
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Spectroscopic studies on the binding of bromocresol purple to different serum albumins and its bilirubin displacing action
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 90, Issue 1, 30 January 2008, Pages 1–7
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
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Any Questions? feel free to contact us