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In vitro study on the binding of herbicide glyphosate to human serum albumin by optical spectroscopy and molecular modeling

Paper ID Volume ID Publish Year Pages File Format Full-Text
30406 44477 2008 7 PDF Available
Title
In vitro study on the binding of herbicide glyphosate to human serum albumin by optical spectroscopy and molecular modeling
Abstract

In this study, the interaction mechanism of herbicide glyphosate and human serum albumin (HSA) has been characterized by fluorescence, UV, Fourier transform infrared (FT-IR), Circular dichroism (CD) spectroscopic and molecular modeling methods. The structural characteristics of glyphosate and HSA were probed, and affinity constants were determined under different temperatures. The enthalpy change (ΔH°) and the entropy changes (ΔS°) were calculated to be −21.78 kJ mol−1 and 6.38 Jmol−1 K−1 according to the Van’t Hoff equation. These results indicated that glyphosate binds to HSA mainly by hydrogen bond and hydrophobic interaction can also not be excluded, which is in good agreement with the results from modeling experiment. The average binding distance, r, between the donor (HSA) and the acceptor (glyphosate) was evaluated and found to be 2.89 nm according to the Förster’s theory of non-radiation energy transfer. The alterations of protein secondary structure in the presence of glyphosate were confirmed by the evidences from UV, FT-IR and CD spectroscopes.

Keywords
Glyphosate; Human serum albumin (HSA); Circular dichroism (CD); Fourier transformation infrared spectra (FT-IR); Molecular modeling; Fluorescence
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In vitro study on the binding of herbicide glyphosate to human serum albumin by optical spectroscopy and molecular modeling
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 90, Issue 1, 30 January 2008, Pages 26–32
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us