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Interaction of 7-hydroxyflavone with human serum albumin: A spectroscopic study

Paper ID Volume ID Publish Year Pages File Format Full-Text
30407 44477 2008 8 PDF Available
Title
Interaction of 7-hydroxyflavone with human serum albumin: A spectroscopic study
Abstract

Numerous recent investigations have revealed that various synthetic as well as therapeutically active natural flavonoids possess novel luminescence properties that can serve as highly sensitive monitors for exploring their interactions with relevant physiological targets. Here we report a detailed study on the interactions of the model flavone, 7-hydroxyflavone (7HF) with the plasma protein human serum albumin (HSA), employing electronic absorption, fluorescence (steady state and time resolved) and induced circular dichroism (ICD) spectroscopy. The spectral data indicate that in the protein matrix, the neutral 7HF molecules are predominantly transformed to a conjugate anion (7HFA) by a proton abstraction in the ground state. The protein (HSA) environment induces dramatic enhancements in the fluorescence emission intensity, anisotropy (r) and lifetime (τ) values, as well as pronounced changes in the fluorescence excitation and emission profiles of the fluorophore. Moreover, evidence for efficient Förster type resonance energy transfer (FRET, from tryptophan to 7HFA) is presented, from which we infer that the binding site of 7HF in HSA is proximal (estimated distance, R = 23.6 Å) to the unique tryptophan – 214 residue present in the inter-domain (between IIA and IIIA domains) loop region of the protein. The binding constant (K = 9.44 × 104 M−1) and the Gibbs free energy change (ΔG = −28.33 kJ/mol) for 7HFA–HSA interaction have been estimated from the emission data. Finally, the near-UV circular dichroism (CD) studies show that the electronic transitions of 7HF are strongly perturbed on binding to the chiral host (HSA), leading to the appearance of ICD bands. Implications of these results are discussed.

Keywords
Protein–flavone interaction; 7-Hydroxyflavone; Steady state fluorescence; Fluorescence lifetime; FRET; Induced circular dichroism (ICD)
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Interaction of 7-hydroxyflavone with human serum albumin: A spectroscopic study
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 90, Issue 1, 30 January 2008, Pages 33–40
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us