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Simultaneous saccharification and fermentation of xylo-oligosaccharides manufacturing waste residue for l-lactic acid production by Rhizopus oryzae

Paper ID Volume ID Publish Year Pages File Format Full-Text
3042 148 2015 8 PDF Available
Title
Simultaneous saccharification and fermentation of xylo-oligosaccharides manufacturing waste residue for l-lactic acid production by Rhizopus oryzae
Abstract

•Lactic acid production from XOS manufacturing waste by Rhizopus oryzae was investigated.•Enzymatic hydrolysis of the waste gave high yields of glucose (87.1%) and xylose (64.2%).•Separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) were studied.•34.0 g/L l-lactic acid with a yield of 0.34 g g−1 dry waste residue was obtained in SHF.•Higher lactic acid titer (60.3 g/L) and yield (0.60 g g−1) were achieved in SSF at 10% (w/v) loading.

High substrate cost and low lactic acid yield are the most pressing concerns in fermentative production of l-lactic acid by Rhizopus oryzae. In this study, waste residue from corncob after xylo-oligosaccharides (XOS) manufacturing was used as an alternative abundant, renewable, and inexpensive substrate for l-lactic acid production. After enzymatic hydrolysis, both glucose and xylose in the hydrolysate were converted to 34.0 g L−1 of l-lactic acid, equivalent to a yield of 0.34 g g−1 dry waste residue, by R. oryzae in separate hydrolysis and fermentation. In contrast, a higher l-lactic acid titer (60.3 g L−1) and yield (0.60 g g−1 dry waste residue) were achieved in simultaneous saccharification and fermentation (SSF) with 10% (w/v) substrate loading at 40 °C, demonstrating, for the first time, the feasibility of l-lactic acid production from XOS manufacturing waste residues. The SSF process for l-lactic acid production from XOS waste residues was also demonstrated in a 5-L stirred-tank bioreactor, although further optimization would be necessary.

Keywords
R. oryzae, Rhizopus oryzae; XOS, xylo-oligosaccharides; SHF, separate hydrolysis and fermentation; SSF, simultaneous saccharification and fermentationCellulose; Fermentation; Filamentous fungi; Lactic acid; Rhizopus oryzae; Xylo-oligosaccharides
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Simultaneous saccharification and fermentation of xylo-oligosaccharides manufacturing waste residue for l-lactic acid production by Rhizopus oryzae
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 94, 15 February 2015, Pages 92–99
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us