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In vitro DNA binding studies of the sweetening agent saccharin and its copper(II) and zinc(II) complexes

Paper ID Volume ID Publish Year Pages File Format Full-Text
30428 44478 2014 7 PDF Available
Title
In vitro DNA binding studies of the sweetening agent saccharin and its copper(II) and zinc(II) complexes
Abstract

•Comparative DNA binding studies of saccharin and its complexes.•All compounds bind to DNA.•Na(sac) and Zn-sac act as groove binders.•Cu-sac is a moderate intercalator.•Cu-sac and Zn-sac show weak ability to damage to supercoiled DNA.

The interactions of fish sperm DNA (FS-DNA) with the sodium salt of sweetener saccharin (sacH) and its copper and zinc complexes, namely [M(sac)2(H2O)4]·2H2O (M = CuII or ZnII) were studied by using UV–Vis titration, fluorometric competition, thermal denaturation, viscosity and gel electrophoresis measurements. The intrinsic binding constants (Kb) obtained from absorption titrations were estimated to be 2.86 (±0.06) × 104 M−1 for Na(sac), 6.67 (±0.12) × 104 M−1 for Cu-sac and 4.01 (±0.08) × 104 M−1 for Zn-sac. The Cu-sac complex binds to FS-DNA via intercalation with a KA value of 50.12 (±0.22) × 104 M−1 as evidenced by competitive binding studies with ethidium bromide. Moreover, competition experiments with Hoechst 33258 are indicative of a groove binding mode of Na(sac) and Zn-sac with binding constants of 3.13 (±0.16) × 104 M−1 and 5.25 (±0.22) × 104 M−1, respectively. The spectroscopic measurements indicate a moderate DNA binding affinity of Na(sac) and its metal complexes. The suggested binding modes are further confirmed by the thermal denaturation and viscosity measurements. In addition, Cu-sac and Zn-sac show weak ability to damage to pBR322 supercoiled plasmid DNA.

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Keywords
Saccharin; DNA binding; Copper(II) saccharinate; Zinc(II) saccharinate; UV–Vis spectroscopy; Fluorescence spectroscopy
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In vitro DNA binding studies of the sweetening agent saccharin and its copper(II) and zinc(II) complexes
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 130, 5 January 2014, Pages 115–121
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us