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The fate of anticancer drug, ellipticine in DPPC and DMPC liposomes upon interaction with HSA: A photophysical approach

Paper ID Volume ID Publish Year Pages File Format Full-Text
30429 44478 2014 10 PDF Available
Title
The fate of anticancer drug, ellipticine in DPPC and DMPC liposomes upon interaction with HSA: A photophysical approach
Abstract

•We studied photophysics of ellipticine in liposome–HSA system.•HSA penetrates in liposomes due to hydrophobic interaction.•The penetration of HSA is higher in DPPC liposomes compared to that in DMPC liposomes.•The rotational relaxation time is higher in liposome–HSA complex compared to that in liposomes.

Interaction of anticancer drug, ellipticine with Human Serum Albumin (HSA) and release of this encapsulated drug from two individual liposomes namely 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) upon addition of HSA have been studied by steady state and time resolved fluorescence spectroscopy. It was observed that HSA penetrates into the liposomes through hydrophobic interaction which reduces the packing order of the lipid bi-layer and leads to a quenching in fluorescence intensity of ellipticine. DPPC is more dehydrated hence more hydrophobic due to its higher phase transition temperature (42 °C) as compared to that of DMPC (23 °C). Therefore, HSA exhibits more affinity towards DPPC than it does towards DMPC. The time resolved components revealed that penetration of HSA into liposomes results in migration of the drug molecules from liposomes to hydrophobic pocket of HSA. Incorporation of HSA in both the liposomes increases the rotational relaxation time of ellipticine. The fact confirms that HSA penetrates into the liposome and forms bigger complex.

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Keywords
Ellipticine; Binding; Interaction; Liposomes; HSA; Penetration
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The fate of anticancer drug, ellipticine in DPPC and DMPC liposomes upon interaction with HSA: A photophysical approach
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 130, 5 January 2014, Pages 122–131
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us