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Spectroscopic analysis of the interaction of lomustine with calf thymus DNA

Paper ID Volume ID Publish Year Pages File Format Full-Text
30448 44478 2014 6 PDF Available
Title
Spectroscopic analysis of the interaction of lomustine with calf thymus DNA
Abstract

•Interaction of lomustine with DNA was investigated using FTIR and CD spectroscopic techniques.•FTIR analysis suggests lomustine binding with guanine and cytosine bases of DNA along with weak interaction to backbone.•Circular dichroism spectroscopic results support the partial B–A transition in DNA conformation.

Investigation of drug–DNA interaction is important for understanding the drug action at molecular level and for designing specific DNA targeted drug. Lomustine (CCNU = 1-[2-chloroethyl]-3-cyclohexyl-1-nitroso-urea) is an alkylating antineoplastic nitrosourea derivative, used to treat different types of cancer. In the present study, conformational and structural effects of lomustine on DNA are investigated using different spectroscopic approaches. Different drug/DNA molar ratios are analyzed to determine the binding sites and binding mode of lomustine with DNA. Fourier transform infrared spectroscopic (FTIR) results suggest binding of lomustine with nitrogenous bases guanine and cytosine along with weak interaction to the sugar-phosphate backbone of DNA. Circular dichroism (CD) spectroscopic results show perturbation in the local conformation of DNA upon binding of lomustine with DNA helix. These local conformational changes may act as recognition site for alkylating enzymes that further causes alkylation of DNA. Spectroscopic results confirm the formation of an intermediate stage of DNA that occurs during the transition of B-conformation into A-conformation.

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Keywords
Lomustine; Drug–DNA interaction; FTIR spectroscopy; CD spectroscopy
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Spectroscopic analysis of the interaction of lomustine with calf thymus DNA
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 130, 5 January 2014, Pages 281–286
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us