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Transcriptional response of Enterococcus faecalis to sunlight

Paper ID Volume ID Publish Year Pages File Format Full-Text
30458 44478 2014 8 PDF Available
Title
Transcriptional response of Enterococcus faecalis to sunlight
Abstract

•After 6 h of sun E. faecalis concentrations decreased 6–7 orders of magnitude.•After 12 h of sun E. faecalis transcribe RNA encoding for ribosomal and stress response proteins.•E. faecalis exposed to sunlight in oligotrophic seawater were not able to repair photo-damage.

Microarrays were used to investigate the transcriptional response of Enterococcus faecalis to photostress. E. faecalis are Gram-positive bacteria used as indicators of water quality and have been shown to vary diurnally in response to sunlight. E. faecalis in filtered seawater microcosms were exposed to artificial sunlight for 12 h and then placed in the dark for 12 h. Transcript abundance was measured at 0, 2, 6, 12, and 24 h in the sunlit microcosm and a dark control using microarrays. Culturable E. faecalis concentrations decreased 6–7 orders of magnitude within the first 6 h of light exposure. After 12 h in the dark, no evidence of dark-repair was observed. Expression data collected after 12 h of sunlight exposure revealed a difference in transcript abundance in the light relative to dark microcosms for 35 unique ORFs, 33 ORFs showed increased transcript abundance and 2 ORFs showed reduced transcript abundance. A majority (51%) of the ORFs with increased transcript abundance in the sunlit relative to dark microcosms encoded hypothetical proteins; others were associated with protein synthesis, oxidative stress and DNA repair. Results suggest that E. faecalis exposed to sunlight actively transcribe RNA in response to photostress.

Keywords
Enterococcus; Photoinactivation; Gene expression; Sunlight; Oxidative stress
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 130, 5 January 2014, Pages 349–356
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us